Lightcycler realtime pcr systems application manual. Roche molecular biochemicals lightcycler operators manual. The lightcycler 480 ii with led is suitable for detection systems with different probe types such as hybridization probes or hydrolysis 5nuclease taqman probes. The lightcycler 480 system is a proven highperformance, mediumto highthroughput pcr platform that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation. The lightcycler 480 gene scanning software is intended for general laboratory use in combination with the lightcycler 480 instrument and lightcycler 480 software. This document is intended as a guide to running kasptm genotyping reactions on the roche lightcycler 480 lc480 instrument.
Lightcycler 480 gene scanning software manual the lightcycler 480 gene scanning software scans pcr fragments for the presence of genetic variations e. Instrument package includes lightcycler 480 instrument, lightcycler 480 thermal block unit 96 or 384well, lightcycler 480 software, version 1. It offers fast and accurate analysis that impresses with its intuitive and userfriendly software. Easytouse hotstart reaction mix for pcr using the lightcycler 480 system. Service manual light cycler please note that this is only. The lightcycler 480 software enables users to quickly customize the system to meet different application requirements, from basic manual analysis to automated evaluation of data using the software macro feature. Pcr is performed for extra five cycles relative to the recommendation in the user manual i. It facilitates highly versatile and extensive data analysis to study gene expression and genetic variation. Kits include kapa sybr fast qpcr master mix, a platformspecific library quantification primer premix, and a prediluted. Discover the new lightcycler 96 realtime pcr system from roche. Lightcycler 480 realtime pcr system from roche applied. Find and purchase roche lightcycler 480 products at invitrogen life science technologies.
Aug 06, 2015 roche lightcycler 480 ii real time pcr system for 96 well plates 8856. Preanalytic efficiency the cobas p 480 instrument improves laboratory efficiency by allowing valuable technician time to be used more productively, eliminating repetitive, manual sample handling, improving workflow and reducing risk of contamination, human. Used roche lightcycler480 roche lightcycler480ii384 real. The lightcycler 480 control kit is designed to prove the performance of all components of the lightcycler 480 system, including instrument, software, consumables, generic reagents, and optional devices such as a pipetting. Guide to running kasptm genotyping reactions on the roche. I am using the lightcycler 480 sybr green i master kit from roche, but it is brand new.
The software is compatible with the regulations for electronic records subpart b. A multitude of different layouts is available for configuration. How can i solve the problem of roche lightcycler 480. It enables users to quickly customize the system to meet different application requirements, from basic manual analysis, a wide choice of flexible quantification methods in line with miqe guidelines, to tools for multiple plate analysis.
V license statements for the lightcycler 480 gene scanning software parts of the software used for the lightcycler 480 system are licensed from idaho. Roche product information related to the us market. The lightcycler 480 software provides innovative solutions for various quantitative realtime pcr qpcr analyses. The system features the lightcycler 480 instrument, a versatile, platebased. The kit can be used to verify the lightcycler 480 systems speed, accuracy, and intrawell assay reproducibility. However, roche diagnostics gmbh reserves the right to make any changes necessary without notice as part of ongoing product development. Lightcycler 480 realtime pcr system rapid by nature.
Roche diagnostics gmbh hereby grants to licensee a nonexclusive, single use license to if you had used the lightcycler software 1. In the us our pharmaceuticals are marketed by genentech. Kapa library quantification kits roche sequencing solutions. Create your specific gene expression panel with realtime ready assays of your choice on lightcycler 480 multiwell plates 96 or 384, using the realtime ready configurator. Executed on roche lightcycler 480 realtime pcr system for safety and biohazard guidelines, refer to the safety section in the power sybr green pcr master mix and rtpcr protocol pn 4367218. Lightcycler 480 sybr green i master version june 2005 easytouse hotstart reaction mix for pcr using the lightcycler 480 system. Based on innovative hardware and software, the lightcycler 480 instrument is a modular online pcr device for qualitative or quantitative detection of nucleic acids, genotyping, and mutation analysis. Other areas include viral infections, metabolic, central nervous system disorders and inflammatory diseases.
Any unauthorized modification of the instrument entails the invalidity of the guarantee. For todays academic researcher labs, the new roche lightcycler 96 system is the optimal midsize, midthroughput realtime pcr solution that conveniently delivers the highest standards expected from a lightcycler system. Service manual light cycler please note that this is only a. Lightcycler 480 software for genetic variation analysis 58 lightcycler 480 multiple plate analysis software 59 221020908 realtime pcr application db 41020908 realtime pcr application db 4 006. General lightcycler 480 software user interface conventions. Roche applied science lightcycler 480 realtime pcr system. For in vitro diagnostic applications in combination with roche diagnostic reagent kits use manual a. Lightcycler roche life science realtime ready overview.
Read the msds and follow the handling instructions. Absolute and relative quantification analysis methods, and subtypes of these techniques are implemented in the lightcycler 480 software. Start the lightcycler 480 software by doubleclicking the icon. I prepared the twostep cycling experiment with the following conditions. Warranty information on warranty conditions are specified in the sales contract. Realtime pcr monitoring on the lightcycler, offers a convenient way to identify and measure these loglinear cycles.
Quickly start experiments from readytouse macros or templates for all applications. Roche has brought many highly effective drugs onto the market and is a world leader in innovative cancer drugs. The lightcycler 480 ii is a realtime pcr cycler for mid to high sample throughput. The lightcycler 96 is a realtime pcr cycler for medium sample throughput. The flow software offers configurable instrumentation setup with the ability to add up to three magna pure 96 instruments and five lightcycler 480 systems. Taqman fast advanced master mix on the roche lightcycler. Roche applied science is delighted to offer biomedical researchers using realtime pcr its support on. Kasp chemistry for allelic discrimination performs well on a roche lc480series machine and this stepbystep protocol will enable users to successfully setup, run and read plates on the lc480. Has anybody used the light cycler 480 system by roche. The lightcycler 480 system features a versatile, platebased, realtime pcr device that supports mono or multicolor applications. The complete security suite for all your datacommunication needs invisible secrets encryption software 4 not only encrypts your data and files for safe keeping or for secure transfer across the net, it also hides them in places that on the surface appear totally innocent, such as picture or sound files, or web pages.
Roche assays for the cobas 4800 system help deliver consistently reliable results because. Here is a roche lightcycler 480 ii96 pcr predecessor to. Roche lightcycler 480 ii real time pcr system for 96 well. Roche diagnostics 04887301001 lightcycler 480 probes master 10 x 5 ml 2x conc. Lightcycler software 4 software free download lightcycler. Roche applied sciences cuttingedge gene analysis technology is now available in a. Additional features of the lightcycler 480 pcr system. Based on unique lightcycler algorithms, the sophisticated lightcycler 480. This operators manual contains all the necessary technical information required to place the lightcycler instrument in operation and to carry out practical anal yses. Roche lightcycler 480 operators manual department of cell.
Roche diagnostics 04707494001 lightcycler 480 probes master 5 x 1 ml 2x conc. Aug 23, 20 for todays academic researcher labs, the new roche lightcycler 96 system is the optimal midsize, midthroughput realtime pcr solution that conveniently d. The benchtop instrument is easily customizable to meet changing user requirements, and can be integrated into everyday use as an automated highthroughput solution. Disclaimer the text above is not a piece of advice to uninstall lightcycler 480 sw 1.
Used roche lightcycler 480ii96 roche lightcycler 480ii96. However, roche diagnostics gmbh reserves the right to make any changes necessary. Roche applied sciencelightcycler 480 instrumentoperators manual16pt20pt software wenig textimago version medium 1. Roche diagnostics lightcycler 480 probes master lightcycler 480 probes master. Here is a roche lightcycler 480 ii96 pcr unit2 predecessor to. The lightcycler 480 control kit is designed as a tool to confirm realtime pcr and melting curve analysis performance of the lightcycler 480 instrument. This roche lightcycler 480 ii96 pcr w thermocycler. Combined with the appropriate software modules, snp analysis with hybprobe probes, and quantification with hydrolysis probes in a. Amplify your success in medium and highthroughput realtime pcr applications. Roche diagnostics lightcycler 480 probes master lightcycler. Used roche lightcycler roche lightcycler 480ii96 pcr. Rely on the highly adaptable lightcycler 480 system and related software, kits, disposables, reagents, and probes to perform gene expression analysis, snp genotyping, and mutation scanning via high resolution melting hrm.
I have a problem with the analysis in lightcycler 480 software. Featuring easytouse software and a simple and intuitive interface, you can keep your preanalytic processes under complete control. Disclaimer of license no rights to perform the polymerase chain reaction pcr process, including homogeneous or realtime pcr methods, covered by patents owned by roche molecular sys. It accommodates multiple sample types and flexible batch sizes and provides an easytouse software interface that can make operation simple even for lab techs with no molecular diagnostics experience. Combined with the appropriate software modules, snp analysis with hybprobe probes, and quantification with hydrolysis. This roche lightcycler 480 ii96 pcr w thermocycler 96s instrument is in good working order. Roche lightcycler 480 ii real time pcr system for 96 well plates 8856 2. Contact your roche representative for further information. It enables users to quickly customize the system to meet different application requirements, from basic manual analysis, a wide choice of flexible quantification methods in line with miqe guidelines. See your instruments user manual for detailed instructions on how to configure the run method. Used roche lightcycler 480ii96 roche lightcycler 480ii.
Enter the username and assigned password to log in to the lightcycler 480 software. For more information, please consult chapter d, section 4. Discover the new lightcycler 96 realtime pcr system from roche duration. Roche lightcycler 480 ii real time pcr system for 96 well plates 8856. Make sure the correct thermal block cycler 96well for f plate or 384well block format for g plate is in place. System component 1 lightcycler instrument sample carousel for o 1. Overview of the magna pure 96 system ivd from roche duration. Used roche lightcycler480 roche lightcycler480ii384.
Lightcycler 480 realtime pcr system genequantification. Wear appropriate protective eyewear, clothing, and gloves. Lightcycler 480 gene scanning software roche life science. The software shifts the imported standard curve based on how the reference standard compares to the previously run concentration. The cycler has 4 detection channels, which detect hydrolysis 5nuclease taqman probes either as single color or multiplex samples. Customizable modular system concept building on the principles of the wellestablished, performanceproven lightcycler software 4. Lightcycler software 4, free lightcycler software 4 software downloads. Roche lightcycler 480 ii384 pcr thermocycler 384s block cycler cover dell latitude i5 running windows 7 pro with lightcycler 480 software ver1. Here is a roche lightcycler 480 ii96 pcr w thermocycler 96s predecessor to. The lightcycler 480 software uses unique roche algorithms for highly accurate and robust automated data analysis.
Roche diagnostics lightcycler 480 probes master manufacturer. How can i solve the problem of roche lightcycler 480 error. A pentium desktop pc is supplied with the instrument. Benefit from cuttingedge thermal block and optical technology that leads to excellent welltowell homogeneity and maximized detection. Overall, the flow software when integrated with the laboratories lis, reduces the need for timeintensive paper reports and sample work lists, enables process tracking and automates. An ideal combination of accuracy, temperature homogeneity, and reproducibility, matched with powerful software so intuitive that it is accessible to any user. Using a qpcrbased solution, kapa library quantification kits provide accurate and reliable quantification of libraries prepared for sequencing on illumina and iontorrent platforms, across a wide range of library types, concentrations, fragment distributions and gc content.